Anti-inflammatory and Antimicrobial Potential of Three Natural Polyketides Isolated from Endophytic Fungus Phomopsis sp CAM212 against to Dysenteric Causing Pathogens

Aims: The present work aimed to evaluate the anti-amoebic, antibacterial, and anti-inflammatory potential of three natural polyketides from Phomopsis sp. CAM212.
 Study Design: Clinical isolates E.histolytica, E.coli ATCC25922 strain, primary peritoneal mouse macrophages were used.
 Places Duration Study: Laboratory Pharmacology Toxicology, Medical Microbiology, Faculty Science, University Yaounde 1 between May December 2022.
 Methodology: During this work, we evaluated ability sp inhibit growth germs responsible for amoebic bacillary dysentery. First, anti-amoebic activity was carried out on clinical E. histolytica in polyxenic culture. Subsequently, antibacterial a strain coli ATCC25922. Finally, potentials culture SC activated through inhibition nitric oxide (NO) production, activation phosphatase alcaline (ALP) 5-lipoxygenase (5-LOX).
 Results: It emerges that among compounds, phomopsinin B, presented highest (84.4 % after 72h) (MIC=12.5µg/mL MBC/MIC=2). Phomopsini A phomopsini acetate showed moderate anti potentials. However, all these activities remain lower than metronidazole ciprofloxacin (90% 72h; MIC=0.72µg/mL MBC/MIC=4). tested compounds nontoxic macrophages. Phomopsinin B exhibited great NO production (IC50=1.72±0.91µg/mL); inihibition 5-LOX (IC50=36.97±7.12µg/mL) ALP (IC50=0.13±0.01µg/mL) as compared Baicalin standard. baicalin.
 Conclusion: Ultimately, tested, best ant-inflammatory similar respective standards within limits tests out.